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人沙眼衣原體酶免試劑盒,(CT)ELISA檢測試劑盒,英文名:CT ELISA Kit,英文縮寫:CT ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人沙眼衣原體ELISA檢測試劑盒,CT 檢測試劑盒,CT 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人沙眼衣原體酶免試劑盒,(CT)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
凋亡蛋白酶激活因子1(APAF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)48T/96TY08985Homo sapiens (Human)
V-Myb髓細(xì)胞組織增生病毒癌基因同源物(MYB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Myb Myeloblastosis Viral Oncogene Homolog (MYB)48T/96TY08986Homo sapiens (Human)
70kDa熱休克蛋白4(HSPA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 4 (HSPA4)48T/96TY08987Homo sapiens (Human)
泛素連接酶E3A(UBE3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin Protein Ligase E3A (UBE3A)48T/96TY08988Homo sapiens (Human)
多聚免疫球蛋白受體(PIGR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Polymeric Immunoglobulin Receptor (PIGR)48T/96TY08989Homo sapiens (Human)
促胰液素(SCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretin (SCT)48T/96TY08990Homo sapiens (Human)
促胰液素受體(SCTR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretin Receptor (SCTR)48T/96TY08991Homo sapiens (Human)
ⅡD組磷脂酶A2(PLA2G2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Group IID (PLA2G2D)48T/96TY08992Homo sapiens (Human)
S100鈣結(jié)合蛋白A13(S100A13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A13 (S100A13)48T/96TY08993Homo sapiens (Human)
通用轉(zhuǎn)錄因子ⅡH肽1(GTF2H1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for General Transcription Factor IIH, Polypeptide 1 (GTF2H1)48T/96TY08994Homo sapiens (Human)
S100鈣結(jié)合蛋白A12(S100A12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A12 (S100A12)48T/96TY08995Homo sapiens (Human)
70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A)48T/96TY08996Homo sapiens (Human)
70kDa熱休克蛋白9(HSPA9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 9 (HSPA9)48T/96TY08997Homo sapiens (Human)
甘丙肽(GAL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Galanin (GAL)48T/96TY08998Homo sapiens (Human)
線粒體超氧化物歧化酶(SOD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Superoxide Dismutase 2, Mitochondrial (SOD2)48T/96TY08999Homo sapiens (Human)
腸堿性磷酸酶(ALPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase, Intestinal (ALPI)48T/96TY09000Homo sapiens (Human)
早期生長應(yīng)答因子4(EGR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Early Growth Response Protein 4 (EGR4)48T/96TY09001Homo sapiens (Human)
35kDa核孔蛋白(NUP35)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 35kDa (NUP35)48T/96TY09002Homo sapiens (Human)
37kDa核孔蛋白(NUP37)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 37kDa (NUP37)48T/96TY09003Homo sapiens (Human)
谷胱甘肽S轉(zhuǎn)移酶κ1(GSTκ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Kappa 1 (GSTk1)48T/96TY09004Homo sapiens (Human)
谷胱甘肽S轉(zhuǎn)移酶π1(GSTπ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Pi (GSTp)48T/96TY09005Homo sapiens (Human)
肝腎骨堿性磷酸酶(ALPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)48T/96TY09006Homo sapiens (Human)
骨涎蛋白(BSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Sialoprotein (BSP)48T/96TY09007Homo sapiens (Human)
凝血因子ⅩⅢA1肽(F13A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XIII A1 Polypeptide (F13A1)48T/96TY09008Homo sapiens (Human)
腦指蛋白(BFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Brain Finger Protein (BFP)48T/96TY09009Homo sapiens (Human)
核基質(zhì)蛋白4(NMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Matrix Protein 4 (NMP4)48T/96TY09010Homo sapiens (Human)
胎兒腎鈣黏蛋白(KCAD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin, Fetal Kidney (KCAD)48T/96TY09011Homo sapiens (Human)
花生四烯酸(AA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arachidonic Acid (AA)48T/96TY09012General
低密度脂蛋白(LDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Low Density Lipoprotein (LDL)48T/96TY09013Homo sapiens (Human)
肌細(xì)胞生成素(MYOG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myogenin (MYOG)48T/96TY09014Homo sapiens (Human)
85kDa核孔蛋白(NUP85)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 85kDa (NUP85)48T/96TY09015Homo sapiens (Human)
107kDa核孔蛋白(NUP107)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 107kDa (NUP107)48T/96TY09016Homo sapiens (Human)
中性粒細(xì)胞堿性磷酸酶(NAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neutrophil Alkaline Phosphatase (NAP)48T/96TY09017Homo sapiens (Human)
甲胎蛋白異質(zhì)體1(αFPL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein Lens Culinaris Agglutinin 1 (aFPL1)48T/96TY09018Homo sapiens (Human)
甲胎蛋白異質(zhì)體2(αFPL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-Fetoprotein Lens Culinaris Agglutinin 2 (aFPL2)48T/96TY09019Homo sapiens (Human)
成纖維細(xì)胞生長因子23(FGF23)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 23 (FGF23)48T/96TY09020Homo sapiens (Human)
CCAAT增強(qiáng)子結(jié)合蛋白γ(CEBPγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CCAAT/Enhancer Binding Protein Gamma (CEBPg)48T/96TY09021Homo sapiens (Human)
補(bǔ)體1q(C1q)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement 1q (C1q)48T/96TY09022Homo sapiens (Human)
凝血因子Ⅴ(F5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor V (F5)48T/96TY09023Homo sapiens (Human)
凝血因子Ⅴ(F5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor V (F5)48T/96TY09024Rattus norvegicus (Rat)
凝血因子ⅩⅢ(F13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XIII (F13)48T/96TY09025Homo sapiens (Human)
凝血因子ⅩⅢ(F13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XIII (F13)48T/96TY09026Rattus norvegicus (Rat)
凝血因子Ⅻ(F12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XII (F12)48T/96TY09027Homo sapiens (Human)
凝血因子Ⅻ(F12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XII (F12)48T/96TY09028Rattus norvegicus (Rat)
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY09029Cavia (Guinea pig )
三葉因子2(TFF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY09030Homo sapiens (Human)
三葉因子2(TFF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trefoil Factor 2 (TFF2)48T/96TY09031Sus scrofa; Porcine (Pig)
膽囊收縮素(CCK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholecystokinin (CCK)48T/96TY09032Sus scrofa; Porcine (Pig)
二胺氧化酶(DAO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diamine Oxidase (DAO)48T/96TY09033Sus scrofa; Porcine (Pig)
表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Surfactant Associated Protein B (SPB)48T/96TY09034Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。