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小鼠糖蛋白130酶免試劑盒,(gp130)ELISA檢測試劑盒,英文名:gp130 ELISA Kit,英文縮寫:gp130 ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠糖蛋白130ELISA檢測試劑盒,gp130 檢測試劑盒,gp130 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠糖蛋白130酶免試劑盒,(gp130)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
基質細胞衍生因子1(SDF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)48T/96TY10285Mus musculus (Mouse)
轉化生長因子α(TGFα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Alpha (TGFa)48T/96TY10286Homo sapiens (Human)
轉化生長因子β1(TGFβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)48T/96TY10287Homo sapiens (Human)
轉化生長因子β1(TGFβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)48T/96TY10288Mus musculus (Mouse)
轉化生長因子β1(TGFβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)48T/96TY10289Rattus norvegicus (Rat)
免疫球蛋白樣EGF樣域酪氨酸激酶1(Tie1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)48T/96TY10290Homo sapiens (Human)
內皮細胞TEK酪氨酸激酶(Tie2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2)48T/96TY10291Homo sapiens (Human)
組織金屬蛋白酶抑制因子2(TIMP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)48T/96TY10292Homo sapiens (Human)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY10293Homo sapiens (Human)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY10294Homo sapiens (Human)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY10295Homo sapiens (Human)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY10296Mus musculus (Mouse)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY10297Rattus norvegicus (Rat)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY10298Homo sapiens (Human)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY10299Mus musculus (Mouse)
血小板生成素(TPO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thrombopoietin (TPO)48T/96TY10300Homo sapiens (Human)
血小板生成素(TPO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thrombopoietin (TPO)48T/96TY10301Mus musculus (Mouse)
尿激酶型纖溶酶原激活因子(uPA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plasminogen Activator, Urokinase (uPA)48T/96TY10302Homo sapiens (Human)
尿激酶型纖溶酶原激活因子受體(uPAR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR)48T/96TY10303Homo sapiens (Human)
細胞間粘附分子5(ICAM5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 5 (ICAM5)48T/96TY10304Homo sapiens (Human)
細胞間粘附分子5(ICAM5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 5 (ICAM5)48T/96TY10305Mus musculus (Mouse)
血管內皮生長因子121(VEGF121)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor 121 (VEGF121)48T/96TY10306Homo sapiens (Human)
血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY10307Rattus norvegicus (Rat)
血管內皮生長因子B(VEGFB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor B (VEGFB)48T/96TY10308Homo sapiens (Human)
血管內皮生長因子C(VEGFC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor C (VEGFC)48T/96TY10309Homo sapiens (Human)
血管內皮生長因子D(VEGFD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor D (VEGFD)48T/96TY10310Homo sapiens (Human)
癌胚抗原(CEA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carcinoembryonic Antigen (CEA)48T/96TY10311Homo sapiens (Human)
激肽釋放酶3(KLK3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 3 (KLK3)48T/96TY10312Homo sapiens (Human)
糖抗原125(CA125)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbohydrate Antigen 125 (CA125)48T/96TY10313Homo sapiens (Human)
糖抗原15-3(CA15-3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbohydrate Antigen 15-3 (CA15-3)48T/96TY10314Homo sapiens (Human)
糖抗原19-9(CA19-9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbohydrate Antigen 19-9 (CA19-9)48T/96TY10315Homo sapiens (Human)
糖抗原242(CA242)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carbohydrate Antigen 242 (CA242)48T/96TY10316Homo sapiens (Human)
激活素AB(ACVAB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Activin AB (ACVAB)48T/96TY10317Mus musculus (Mouse)
鱗狀細胞癌抗原2(SCCA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Squamous Cell Carcinoma Antigen 2 (SCCA2)48T/96TY10318Homo sapiens (Human)
α2-纖溶酶抑制因子(α2PI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-2-Plasmin Inhibitor (a2PI)48T/96TY10319Mus musculus (Mouse)
組織多肽抗原(TPA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Polypeptide Antigen (TPA)48T/96TY10320Homo sapiens (Human)
泛素(Ub)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY10321Mus musculus (Mouse)
胃蛋白酶原A(PGA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pepsinogen A (PGA)48T/96TY10322Homo sapiens (Human)
胃蛋白酶原C(PGC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pepsinogen C (PGC)48T/96TY10323Homo sapiens (Human)
前列腺素E合酶(PTGES)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Prostaglandin E Synthase, Microsomal (PTGES)48T/96TY10324Homo sapiens (Human)
前列腺酸性磷酸酶(ACP3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Acid Phosphatase 3, Prostatic (ACP3)48T/96TY10325Homo sapiens (Human)
熱休克轉錄因子4(HSF4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock Transcription Factor 4 (HSF4)48T/96TY10326Rattus norvegicus (Rat)
轉化生長因子β1(TGFβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)48T/96TY10327Canis familiaris; Canine (Dog)
髓鞘型脂肪酸結合蛋白(FABP8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 8, Myelin (FABP8)48T/96TY10328Homo sapiens (Human)
Ⅲ型膠原(COL3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type III (COL3)48T/96TY10329Homo sapiens (Human)
Ⅳ型膠原(COL4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Type IV (COL4)48T/96TY10330Homo sapiens (Human)
中性粒細胞彈性蛋白酶(ELA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Elastase 2, Neutrophil (ELA2)48T/96TY10331Mus musculus (Mouse)
透明質酸(HA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hyaluronic Acid (HA)48T/96TY10332General
氨基己糖苷酶Aα(HEXα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hexosaminidase A Alpha (HEXa)48T/96TY10333Mus musculus (Mouse)
半乳糖苷酶β(GLβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Galactosidase Beta (GLb)48T/96TY10334Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。